As described in experimental question E2 and also in Chapter 20, the technique of Northern blotting can be used to detect the transcription of RNA. Draw the results you would expect from a Northern blot if bacteria were grown in media that contained lactose (and no glucose) but had the following mutations:
Lane 1. Normal strain
Lane 2. Strain with a mutation that inactivates the lac repressor
Lane 3. Strain with a mutation that prevents allolactose from binding to the lac repressor
Lane 4. Strain with a mutation that inactivates CAP’
How would your results differ if these bacterial strains were grown in media that did not contain lactose or glucose?
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