The protein known as tyrosinase is needed to make certain types of pigments. Tyrosinase is composed of a single polypeptide with 511 amino acids. Because each amino acid weighs 120 Da (on average), the molecular mass of this protein is approximately 61,300 Da, or 61.3 kDa. People who carry two defective copies of the tyrosinase gene have the condition known as albinism. They are unable to make pigment in the skin, eyes, and hair. Western blotting can be used to detect proteins that are translated from a particular mRNA. This method is described in Chapter 20 and also in experimental question E5. Skin samples were collected from a pigmented individual (lane 1) and from three unrelated albino individuals (lanes 2, 3, and 4) and subjected to a Western blot analysis using an antibody that recognizes tyrosinase. Explain the possible cause of albinism in the three albino individuals.
The protein known as tyrosinase is needed to make certain
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