In Chapter 8, Figure 8.7 describes the evolution of the globin gene family. All genes in this family are homologous to each other, though the degree of sequence similarity varies depending on the time of divergence. Genes that have diverged more recently have sequences that are more similar. For example, the α1 and α2 genes have DNA sequences that are more similar to each other than to the α1 and ξ genes. In a Southern blotting experiment, the degree of sequence similarity can be discerned by varying the, stringency of hybridization. At high temperature (i.e., high stringency), the probe recognizes genes that are only a perfect or very close match. At a lower temperature, however, homologous genes with lower degrees of similarities can be detected because slight mismatches are tolerated. If a Southern blot was conducted on a sample of human chromosomal DNA and a probe was used that was a perfect match to the (β-globin gene, rank the following genes (from those that are detected at high stringency down to those that are only detected at low stringency) as they would appear in a Southern blot experiment: Mb, α1, (β, γA, (, and e.
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